Lifetech細(xì)胞計(jì)數(shù)儀*

Lifetech細(xì)胞計(jì)數(shù)儀*

2018年1月份螞蟻淘新到一批Thermofisher/AMQAF1000/Countess II FL細(xì)胞計(jì)數(shù)儀現(xiàn)貨

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4000-520-616

Thermofisher/AMQAF1000/Countess II FL細(xì)胞計(jì)數(shù)儀現(xiàn)貨

產(chǎn)品介紹：

全新的Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀是一款配備了*進(jìn)的光學(xué)和圖像分析軟件，可以快速對(duì)懸液之中的細(xì)胞進(jìn)行評(píng)估的臺(tái)式細(xì)胞分析平臺(tái)。現(xiàn)如今，隨著其具備了熒光應(yīng)用能力——明場(chǎng)和兩路熒光光學(xué)通道，研究人員可以用之進(jìn)行細(xì)胞計(jì)數(shù)、監(jiān)測(cè)熒光蛋白表達(dá)以及測(cè)定細(xì)胞活力。

Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀開箱

• 靈活—可針對(duì)您的應(yīng)用選擇熒光濾光片
• *—可選配可重復(fù)使用的計(jì)數(shù)玻片，有助于節(jié)省耗材成本
• 精確—可自動(dòng)對(duì)焦，提升了精確度，減少了差異性
• 簡(jiǎn)單—采用易于使用的觸摸屏界面
   

全光譜熒光檢測(cè)

Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀具有兩路熒光通道。其所采集的熒光色通過插入單獨(dú)的光立方進(jìn)行測(cè)定。Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀提供有超過15種光立方可供選擇，檢測(cè)不受表達(dá)GFP和RFP的細(xì)胞的限制。對(duì)于轉(zhuǎn)染了RFP表達(dá)載體，和藍(lán)色核復(fù)染的細(xì)胞，，您是否希望測(cè)定其轉(zhuǎn)染效率？沒問題！您只需加入和RFP和DAPI光立方，即可對(duì)整個(gè)細(xì)胞群之中表達(dá)RFP融合的細(xì)胞進(jìn)行高效地計(jì)數(shù)，同時(shí)還可精確地測(cè)定轉(zhuǎn)染效率。

通過兩種顏色熒光進(jìn)行細(xì)胞計(jì)數(shù)。將10 μL采用CellLight® Histone 2B-RFP轉(zhuǎn)導(dǎo)并采用NucBlue® Live ReadyProbes® 試劑染色的Hela細(xì)胞加入到腔室載玻片上，然后插入到配備了DAPI和RFP光立方的Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀上。新Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀的分割和計(jì)數(shù)算法可以根據(jù)細(xì)胞的熒光性質(zhì)對(duì)其進(jìn)行計(jì)數(shù)。在本實(shí)驗(yàn)中，細(xì)胞群之中86%的Hela細(xì)胞被

Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀所采用的EVOS®光立方。

Countess® II FL全自動(dòng)細(xì)胞計(jì)數(shù)儀的熒光檢測(cè)技術(shù)的核心是的EVOS® LED光立方。每個(gè)光立方含有一個(gè)LED、發(fā)光光學(xué)器件和濾光片。光立方具有插拔功能，可由用戶更換，并通過Countess®系統(tǒng)進(jìn)行自動(dòng)配置。這些類型廣泛的光立方為眾多熒光研究應(yīng)用（如細(xì)胞活力分析）提供了更大的靈活性。

See the entire line of compatible EVOS® light cubes

The Countess® II FL Automated Cell Counter is a benchtop instrument designed and manufactured by Invitrogen, which performs cell counting and viability measurements in concert with trypan blue stain.  The Countess™ provides fast, easy and accurate cell counting without using a hemocytometer, thus eliminating the tedium and subjectivity of manual cell counting.
 
GIBCO® brand TrypLE™ Select and TrypLE™ Express are recombinant serine protease enzymes of high purity, which contain no components of animal origin.  TrypLE is designed to remove adherent cells from the surface of plastic flasks and plates in a gentler manner.  In addition, it is room temperature stable and a direct replacement for trypsin.

• This protocol details the procedure for obtaining accurate cell counts using TrypLE cell dissociation products with the Countess® II FL Automated Cell Counter.